9FMI

PsiM N247A in complex with SAH and norbaeocystin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-15
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.6888
Spacegroup name	P 21 21 21
Unit cell lengths	49.299, 78.817, 83.935
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.780 - 0.900
R-factor	0.1422
Rwork	0.142
R-free	0.15330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.005
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487:000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.780	0.930
High resolution limit [Å]	0.900	0.900
Number of reflections	223262	13486
<I/σ(I)>	17.21
Completeness [%]	92.5
Redundancy	11.1
CC(1/2)	0.999	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	100 mM Tris HCl pH 8.5, 20% PEG 8000, 200 mM MgCl2