9FLN
Crystal structure of the C-terminal domain of VldE H373A from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 3 |
| Unit cell lengths | 59.139, 59.139, 32.481 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.569 - 1.140 |
| Rwork | 0.107 |
| R-free | 0.12780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.979 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.570 | 1.160 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.052 | 1.879 |
| Rmeas | 0.057 | |
| Number of reflections | 46365 | 2322 |
| <I/σ(I)> | 18.8 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 9.5 |
| CC(1/2) | 0.999 | 0.401 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 291 | 100 mM Hepes pH 7.2, 50 mM Cadmium sulfate and 0.6 M Sodium acetate |
