9FLM
Crystal structure of the C-terminal domain of VldE from Streptococcus pneumoniae in a catalytically competent conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 3 |
| Unit cell lengths | 57.111, 57.111, 32.674 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.556 - 1.500 |
| Rwork | 0.166 |
| R-free | 0.19870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.008 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.560 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.223 | 1.759 |
| Rpim | 0.109 | 0.864 |
| Number of reflections | 18946 | 921 |
| <I/σ(I)> | 6.1 | 1.3 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 10.2 | 10.2 |
| CC(1/2) | 0.986 | 0.715 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 100 mM Hepes pH 7.5, 50 mM Cadmium sulfate and 1 M of Sodium acetate, soaked with 20 mM EDTA |
