9FLL
Crystal structure of the C-terminal domain of VldE from Streptococcus pneumoniae containing a zinc atom at the binding site
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.2820 |
| Spacegroup name | P 3 |
| Unit cell lengths | 57.177, 57.177, 32.397 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.710 - 2.800 |
| R-factor | 0.246 |
| Rwork | 0.237 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SPR1875 C-TERMINAL DOMAIN |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.687 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.400 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.137 | 0.555 |
| Number of reflections | 5694 | 444 |
| <I/σ(I)> | 6.4 | 1.7 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 100 mM HEPES, PH 7.5, 50 mM CADMIUM SULFATE, 1 M SODIUM ACETATE, 5 mM EDTA |
