9FLK
Crystal structure of the C-terminal domain of VldE from Streptococcus pneumoniae containing two zinc atoms at the binding site
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.28 |
| Spacegroup name | P 3 |
| Unit cell lengths | 59.121, 59.121, 32.676 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.850 |
| Rwork | 0.213 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SPR1875 C-TERMINAL DOMAIN |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.680 | 1.850 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.151 | 1.308 |
| Number of reflections | 10367 | 684 |
| <I/σ(I)> | 8.9 | 1.3 |
| Completeness [%] | 94.8 | 100 |
| Redundancy | 9.7 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 100 mM TRIS-HCL, pH 8.5, 50 mM CADMIUM SULFATE, 1 M SODIUM ACETATE |
