9FL1
Apo Glyceraldehyde 3-phosphate Dehydrogenase (GapA) from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-21 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.160, 100.617, 97.792 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 95.346 - 1.740 |
| Rwork | 0.150 |
| R-free | 0.19950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.642 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.400 | 1.820 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.161 | 0.534 |
| Number of reflections | 147140 | 7357 |
| <I/σ(I)> | 19 | 2.2 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 300 | 17% w/v PEG8000, 100mM Hepes, 12mg/ml protein |
