9FKL

The structure of glycosynthase IXT6 (E241G mutant), the intracellular xylanase of G.proteiniphilus T-6 in complex with xylobiose-F and xylohexaose-F molecules

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-10
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	169.014, 80.526, 79.074
Unit cell angles	90.00, 91.99, 90.00

Refinement procedure

Resolution	40.260 - 2.400
R-factor	0.1558
Rwork	0.153
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.907
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.260	2.480
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	0.520
Rmeas	0.100	0.610
Rpim	0.050	0.310
Number of reflections	41609	4308
<I/σ(I)>	12.1	2.4
Completeness [%]	100.0
Redundancy	5
CC(1/2)	0.990	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2M Sodium acetate and 0.1M Sodium cacodylate buffer at pH 6.5.