9FKK
The structure of glycosynthase IXT6 (E241G mutant), the intracellular xylanase of G.proteiniphilus T-6 in complex with xylobiose-F and xylotetraose-F molecules
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-10 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.924, 81.458, 79.620 |
| Unit cell angles | 90.00, 91.65, 90.00 |
Refinement procedure
| Resolution | 40.730 - 2.400 |
| R-factor | 0.1697 |
| Rwork | 0.167 |
| R-free | 0.21050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.952 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.730 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.610 |
| Rmeas | 0.130 | 0.710 |
| Rpim | 0.060 | 0.370 |
| Number of reflections | 42632 | 4446 |
| <I/σ(I)> | 9.8 | 2.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 4.7 | |
| CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2M Sodium acetate and 0.1M Sodium cacodylate buffer at pH 6.5. |
