9FKJ
The structure of glycosynthase IXT6 (E241G mutant), the intracellular xylanase of G.proteiniphilus T-6 in complex with two xylobiose-F molecules
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.87 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.165, 80.602, 79.307 |
| Unit cell angles | 90.00, 92.22, 90.00 |
Refinement procedure
| Resolution | 79.250 - 2.000 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.19790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.882 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.250 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.490 |
| Rmeas | 0.090 | 0.580 |
| Rpim | 0.050 | 0.300 |
| Number of reflections | 71422 | 4614 |
| <I/σ(I)> | 9.3 | 2.2 |
| Completeness [%] | 99.2 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.990 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2M Sodium acetate and 0.1M Sodium cacodylate buffer at pH 6.5. |
