9FK9
The structure of XT6 from G.proteiniphilus T-6: The E265G/Q238A/W241A mutant
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-12 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 89.263, 61.817, 110.576 |
| Unit cell angles | 90.00, 105.24, 90.00 |
Refinement procedure
| Resolution | 30.910 - 2.700 |
| R-factor | 0.1973 |
| Rwork | 0.194 |
| R-free | 0.26420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.910 | 2.790 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.270 | 0.560 |
| Rmeas | 0.310 | 0.670 |
| Rpim | 0.140 | 0.340 |
| Number of reflections | 16125 | 2110 |
| <I/σ(I)> | 4.6 | 2.1 |
| Completeness [%] | 99.6 | |
| Redundancy | 4 | |
| CC(1/2) | 0.920 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 14% PEG 5K, 0.1M MES buffer at pH 6.5 and 0.015M Zinc chloride |
