9FK8
The structure of XT6 from G.proteiniphilus T-6: The E265G/N158T mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-05 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 88.871, 61.555, 110.771 |
| Unit cell angles | 90.00, 105.32, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.1526 |
| Rwork | 0.151 |
| R-free | 0.18980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.826 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.100 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.260 |
| Rmeas | 0.070 | 0.320 |
| Rpim | 0.030 | 0.180 |
| Number of reflections | 33811 | 2721 |
| <I/σ(I)> | 16.1 | 3.8 |
| Completeness [%] | 99.7 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.990 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 14% PEG 5K, 0.1M MES buffer at pH 6.5 and 0.02M Zinc chloride |
