9FK4
The structure of glycosynthase XT6 (E265G mutant), the extracellular xylanase of G.proteiniphilus T-6 in complex with xylohexaose-F molecule
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-11 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 89.406, 61.648, 110.293 |
| Unit cell angles | 90.00, 105.47, 90.00 |
Refinement procedure
| Resolution | 35.950 - 1.900 |
| R-factor | 0.1586 |
| Rwork | 0.157 |
| R-free | 0.18830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.846 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.950 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.450 |
| Rmeas | 0.060 | 0.540 |
| Rpim | 0.030 | 0.290 |
| Number of reflections | 45734 | 2929 |
| <I/σ(I)> | 16.5 | 2.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 4.3 | 3 |
| CC(1/2) | 0.990 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.01M Zinc chloride, 17% PEG 5000, and 0.1M MES buffer at pH 6.5. |
