9FJ8
Teth514_1788 1,2-beta-oligomannan phosphorylase in complex with mannotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 137.338, 137.338, 168.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.675 - 2.300 |
| Rwork | 0.169 |
| R-free | 0.18440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.085 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.000 | 2.300 |
| Rmerge | 0.107 | 0.560 |
| Number of reflections | 82187 | 11887 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 8.1 | |
| CC(1/2) | 0.996 | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | 1.4M Ammonium Sulphate 0.2M NaCl 0.1M Na Cacodylate pH 6.5 |
