9FHV
Bacteroides ovatus polysaccharide lyase family 38 (BoPL38) wild type in complex pentasaccharide (ManGul)3 at pH 3.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.679, 89.700, 146.988 |
| Unit cell angles | 90.00, 120.56, 90.00 |
Refinement procedure
| Resolution | 48.990 - 2.340 |
| R-factor | 0.1981 |
| Rwork | 0.196 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.142 |
| Data reduction software | XDS (jan 10, 2022) |
| Data scaling software | XSCALE (jan 10, 2022) |
| Phasing software | PHASER (1.21_5207) |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.990 | 2.370 |
| High resolution limit [Å] | 2.339 | 2.340 |
| Number of reflections | 93431 | 3000 |
| <I/σ(I)> | 9.38 | |
| Completeness [%] | 96.4 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.995 | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 294.15 | 18% (W/V) PEG3350, 0.2 M (NH4)2SO4 and 0.1 M citric acid pH 3.5 |
