9FHO

Crystal structure of the arginine kinase Der p 20_like (putative isoform)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-05
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.978564
Spacegroup name	P 1 21 1
Unit cell lengths	48.561, 61.894, 61.769
Unit cell angles	90.00, 96.44, 90.00

Refinement procedure

Resolution	48.250 - 1.800
Rwork	0.185
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.451
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.250	48.250	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.086	0.085	0.427
Rmeas	0.119	0.119	0.582
Rpim	0.081	0.083	0.393
Number of reflections	33156	282	1967
<I/σ(I)>	6.4
Completeness [%]	97.9
Redundancy	3.2	3.3	3.3
CC(1/2)	0.988	0.955	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.05 M Calcium chloride dihydrate, 0.1 M BIS-TRIS pH 6.5, 30% v/v Polyethylene glycol monomethyl ether 550