9FHK

Structure of the F13 protein of Vaccinia virus (P21 crystal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978564321995
Spacegroup name	P 1 21 1
Unit cell lengths	52.350, 94.111, 73.197
Unit cell angles	90.00, 98.94, 90.00

Refinement procedure

Resolution	39.440 - 2.100
R-factor	0.2197
Rwork	0.218
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.463
Data reduction software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.440	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	40522	3297
<I/σ(I)>	11.3
Completeness [%]	99.1
Redundancy	7.1
CC(1/2)	0.998	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20%(w/v) PEG 3350, 0.1M Hepes 7.5, 2%(v/v) Tacsimate (cryoprotected in 20% glycerol)