9FF3
The structure of delta-ScoC, a global regulator protein from Geobacillus kaustophilus T-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 80.082, 80.082, 164.149 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.900 - 3.320 |
| R-factor | 0.2516 |
| Rwork | 0.249 |
| R-free | 0.28460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.623 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 3.430 |
| High resolution limit [Å] | 3.320 | 3.320 |
| Rmerge | 0.110 | 0.130 |
| Rmeas | 0.110 | 0.590 |
| Rpim | 0.026 | 0.620 |
| Number of reflections | 5080 | 992 |
| <I/σ(I)> | 19.1 | 5.3 |
| Completeness [%] | 99.7 | |
| Redundancy | 19.5 | |
| CC(1/2) | 0.990 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 4% PEG 4K, 0.1M Sodium acetate, and 8% MPD |
