9FES
mCherry - Directionality of Optical Properties of Fluorescent Proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.686, 42.294, 56.683 |
| Unit cell angles | 90.00, 108.07, 90.00 |
Refinement procedure
| Resolution | 44.420 - 1.660 |
| R-factor | 0.19933 |
| Rwork | 0.197 |
| R-free | 0.24295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.848 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.071 | 1.140 |
| Rmeas | 0.077 | 1.272 |
| Rpim | 0.029 | 0.546 |
| Total number of observations | 8236 | |
| Number of reflections | 23804 | 1642 |
| <I/σ(I)> | 12.2 | 1.3 |
| Completeness [%] | 95.1 | |
| Redundancy | 6.8 | 5 |
| CC(1/2) | 0.999 | 0.713 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1 M TRIS hydrochloride, 0.2 M Magnesium chloride hexahydrate, 30% w/v Polyethylene glycol 4000 |
