9FER
wasCFP (SG P21) - Directionality of Optical Properties of Fluorescent Proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-21 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.320, 77.750, 96.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.140 - 1.650 |
| R-factor | 0.21138 |
| Rwork | 0.209 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.031 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.690 |
| High resolution limit [Å] | 1.650 | 7.380 | 1.650 |
| Rmerge | 0.097 | 0.032 | 1.014 |
| Rmeas | 0.112 | 0.035 | 1.224 |
| Number of reflections | 105191 | 1185 | 7560 |
| <I/σ(I)> | 9.02 | ||
| Completeness [%] | 97.9 | ||
| Redundancy | 3.9 | ||
| CC(1/2) | 0.996 | 0.999 | 0.535 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 200mM Sodium chloride 100mM TRIS 30% w/v Polyethylene glycol 3350 |
