9FEN

LSSmOrange (P1) - Directionality of Optical Properties of Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	111.900, 51.480, 55.630
Unit cell angles	90.00, 100.50, 90.00

Refinement procedure

Resolution	42.890 - 1.600
R-factor	0.17116
Rwork	0.170
R-free	0.19935
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.841
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.650
High resolution limit [Å]	1.560	4.660	1.560
Rmerge	0.069	0.024	1.551
Rmeas	0.081	0.028	1.833
Number of reflections	85128	3182	13653
<I/σ(I)>	9.98
Completeness [%]	97.9
Redundancy	2.5
CC(1/2)	0.999	0.999	0.277

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	20 % w/v Polyethylene glycol 6,000 100 mM tri-Sodium citrate