9FEM

mNeonGreen - Directionality of Optical Properties of Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-10
Detector	DECTRIS PILATUS3 R 300K
Wavelength(s)	1.541870
Spacegroup name	P 41 21 2
Unit cell lengths	59.501, 59.501, 132.679
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.480 - 2.320
R-factor	0.23325
Rwork	0.230
R-free	0.29365
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	2.367
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.300	6.830	2.300
Rmerge	0.083	0.038	0.487
Rmeas	0.089	0.042	0.546
Number of reflections	11039	498	1645
<I/σ(I)>	15.37
Completeness [%]	98.4
Redundancy	7.1
CC(1/2)	0.998	0.999	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	100 mM sodium HEPES 20% PEG 8000