9FEL

LSSmCherry1 - Directionality of Optical Properties of Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	39.540, 107.980, 58.730
Unit cell angles	90.00, 103.95, 90.00

Refinement procedure

Resolution	39.200 - 1.500
R-factor	0.18979
Rwork	0.189
R-free	0.21321
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.732
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.590
High resolution limit [Å]	1.500	4.470	1.500
Rmerge	0.121	0.041	1.909
Rmeas	0.131	0.044	2.067
Number of reflections	76295	2927	12303
<I/σ(I)>	9.94
Completeness [%]	99.9
Redundancy	6.8
CC(1/2)	0.998	0.999	0.433

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	100mM Bis-Tris 200mM lithium sulfate 25% PEG 3350