9FEJ

Structure of the RNA-dependent RNA polymerase P2 from the bacteriophage Phi8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-09-06
Detector	ADSC QUANTUM 210
Wavelength(s)	0.93930
Spacegroup name	P 61 2 2
Unit cell lengths	96.666, 96.666, 267.586
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	83.710 - 3.000
R-factor	0.2558
Rwork	0.254
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.429
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	267.470	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.251	2.596
Rmeas	0.254	2.641
Rpim	0.040	0.462
Total number of observations	635295	22492
Number of reflections	15667	732
<I/σ(I)>	5.6	0.2
Completeness [%]	100.0
Redundancy	40.5	30.7
CC(1/2)	0.996	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	296	140 mM CaCl2, 14% PEG 3350, 100 mM Tris pH 8