9FDV

Crystal Structure of reduced NuoEF variant R66G(NuoF) from Aquifex aeolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9724
Spacegroup name	P 21 21 21
Unit cell lengths	63.303, 116.215, 189.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.587 - 1.986
Rwork	0.165
R-free	0.20130
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	1.403
Data reduction software	XDS (VERSION Jan 31, 2020 BUILT=20200131)
Data scaling software	Aimless (0.7.15)
Phasing software	REFMAC (5.8.0425)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.587	2.020
High resolution limit [Å]	1.986	1.986
Rpim	0.066	0.381
Number of reflections	96723	4402
<I/σ(I)>	8
Completeness [%]	99.4
Redundancy	4.6
CC(1/2)	0.994	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	281	100 mM BisTris pH 6.3, 1.4 M ammonium sulfate, 100 mM NaCl