9FDD
The crystal structure of full length tetramer CysB from Klebsiella aerogenes in complex with N-acetylserine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | H 3 |
| Unit cell lengths | 187.436, 187.436, 224.662 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.462 - 2.800 |
| Rwork | 0.180 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.753 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.462 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.089 |
| Number of reflections | 72415 |
| <I/σ(I)> | 5.9 |
| Completeness [%] | 99.4 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 12-17% PEG 8000, 0.2 M sodium tartrate, 0.1 M Tris-HCl |
