9FCG

Medicago truncatula 5'-ProFAR isomerase (HISN3) D57N mutant in complex with PrFAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 4
Unit cell lengths	87.790, 87.790, 35.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.630 - 1.540
R-factor	0.1748
Rwork	0.174
R-free	0.19840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.904
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.630
High resolution limit [Å]	1.540	1.540
Rmerge	0.143	1.592
Rmeas	0.149	1.677
Number of reflections	40420	6090
<I/σ(I)>	12.26	1.46
Completeness [%]	98.8	92.9
Redundancy	13.1
CC(1/2)	0.998	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.2 M sodium bromide, 20% PEG 3350, 1.5 M NaCl in reservoir