9FC9

HEN EGG-WHITE Lysozyme incubated at 50% relative humidity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P09 HiPhaX
Synchrotron site	PETRA III, DESY
Beamline	P09 HiPhaX
Temperature [K]	298
Detector technology	PIXEL
Collection date	2022-11-12
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.7749
Spacegroup name	P 43 21 2
Unit cell lengths	79.656, 79.656, 37.327
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.160 - 1.400
R-factor	0.1604
Rwork	0.158
R-free	0.18540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.997
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.160	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	24202	2354
<I/σ(I)>	14.64	1.97
Completeness [%]	99.9
Redundancy	8.5
CC(1/2)	0.998	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	298	1M NaCl, 35% (v/v) ethylene glycol, 20% (w/v) PEG4000, 50 mM sodium acetate pH 3.5