9FBP
Deletion mutant MmChi60
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9171 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.790, 172.829, 47.088 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 47.088 - 1.844 |
| Rwork | 0.154 |
| R-free | 0.21920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.666 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.062 | 0.437 |
| Rmeas | 0.081 | 0.577 |
| Number of reflections | 54492 | 7740 |
| <I/σ(I)> | 10.02 | 1.65 |
| Completeness [%] | 88.8 | 78.1 |
| Redundancy | 2 | 1.8 |
| CC(1/2) | 0.997 | 0.691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.1 M sodium malonate, 0.5% Jeffamine ED-2001 and 0.1 M HEPES at pH 7.0 |
