9FBK

Diheme cytochrome c Kustd1711 from Kuenenia stuttgartiensis, without glycerol cryoprotectant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.887
Spacegroup name	P 21 21 2
Unit cell lengths	62.600, 92.100, 55.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.430 - 1.700
R-factor	0.1963
Rwork	0.194
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.110
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.750
High resolution limit [Å]	1.700	1.700
Rmeas	0.127	0.818
Number of reflections	34601	4955
<I/σ(I)>	5.5
Completeness [%]	94.7
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M CALCIUM ACETATE, 0.1 M IMIDAZOLE/HCL PH 8.0 AND 20% PEG 1K, VAPOR DIFFUSION