9FBG
SARS-CoV-2 Nucleocapsid N-terminal domain (NTD) mutant P151S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 188.111, 108.883, 146.272 |
| Unit cell angles | 90.00, 100.79, 90.00 |
Refinement procedure
| Resolution | 47.750 - 2.540 |
| R-factor | 0.2228 |
| Rwork | 0.221 |
| R-free | 0.26050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.790 |
| Data reduction software | autoPROC (1.1.7) |
| Data scaling software | Aimless (0.7.13) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.580 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Number of reflections | 95472 | 4703 |
| <I/σ(I)> | 11.7 | 1.6 |
| Completeness [%] | 99.8 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.998 | 0.675 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM Sodium HEPES pH 7.5 4.3M Sodium chloride |
