9FA5
Lysozyme structure at room temperature by serial synchrotron crystallography with COC chips
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 295 |
| Detector technology | PIXEL |
| Collection date | 2024-02-23 |
| Detector | PSI JUNGFRAU 4M |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.300, 78.300, 38.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.150 - 1.600 |
| R-factor | 0.19378 |
| Rwork | 0.191 |
| R-free | 0.25057 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | CrystFEL |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.150 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 16156 | 834 |
| <I/σ(I)> | 5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 295 | 6% (w/v) PEG 6000, 0.1 M sodium acetate pH 3.0, 20% (w/v) sodium chloride |
