9F9V
Crystal structure of 892_05174 from Planctomycetota strain 892
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-02 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.390, 62.110, 66.900 |
| Unit cell angles | 68.83, 88.34, 78.92 |
Refinement procedure
| Resolution | 43.520 - 1.570 |
| R-factor | 0.2101 |
| Rwork | 0.209 |
| R-free | 0.23290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.644 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.520 | 36.450 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.040 | 0.710 |
| Number of reflections | 87524 | 87100 |
| <I/σ(I)> | 13.86 | 1.48 |
| Completeness [%] | 95.4 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.990 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 37,5% w/v M1K2230 0.1 M MB2 7.5 pH - Sodium HEPES; MOPS (acid) 0.1 M MCA (complex ingredient) - 25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350 |
