9F9G

Laser excitation effects on BR: Reprocessed Dark from Nogly et al.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	298
Detector technology	PIXEL
Collection date	2017-06-03
Detector	CS-PAD CXI-1
Wavelength(s)	1.3
Spacegroup name	P 63
Unit cell lengths	62.320, 62.320, 111.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.060 - 1.440
R-factor	0.1497
Rwork	0.149
R-free	0.17210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	1.947
Data reduction software	CrystFEL (0.9.1)
Data scaling software	CrystFEL (0.9.1)
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.060	1.480
High resolution limit [Å]	1.440	1.440
Number of reflections	45078	4511
<I/σ(I)>	22.13	0.87
Completeness [%]	100.0
Redundancy	1371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.6	294	100 mM Na/K Phosphate buffer pH 5.6 30 % PEG 2000