9F8Y
Crystal structure of a designed three-motif Respiratory Syncytial Virus immunogen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.843, 106.432, 113.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.670 - 2.300 |
| R-factor | 0.2615 |
| Rwork | 0.260 |
| R-free | 0.28340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.202 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.670 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.770 | |
| Number of reflections | 135054 | 2558 |
| <I/σ(I)> | 9.17 | |
| Completeness [%] | 99.3 | |
| Redundancy | 5.2 | |
| CC(1/2) | 0.996 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M calcium acetate, Tris pH 7.5, and 25 % (v/v) PEG 2000 MME |
