9F8X
Low-dose structure of Marinobacter nauticus nitrous oxide reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-11-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.260, 69.375, 153.188 |
| Unit cell angles | 81.38, 77.92, 88.61 |
Refinement procedure
| Resolution | 23.501 - 1.498 |
| Rwork | 0.130 |
| R-free | 0.15190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.075 |
| Data reduction software | DENZO |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.501 | 1.520 |
| High resolution limit [Å] | 1.498 | 1.498 |
| Rmerge | 0.026 | 0.258 |
| Rpim | 0.026 | 0.258 |
| Number of reflections | 381570 | 17344 |
| <I/σ(I)> | 12.1 | 3 |
| Completeness [%] | 91.4 | 83.4 |
| Redundancy | 2 | 1.9 |
| CC(1/2) | 1.000 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 303 | 15 % PEG 8000 0.6 M NaCl 0.1 M imidazole/malate protein concentration: 14 mg/ml |
