9F8U
Structure of the mouse 8-oxoguanine DNA Glycosylase mOGG1 in complex with ligand TH7399
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.371, 81.769, 169.985 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.700 - 2.500 |
| Rwork | 0.237 |
| R-free | 0.29670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.920 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.700 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 40082 | 4445 |
| <I/σ(I)> | 19.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.8 | |
| CC(1/2) | 1.000 | 0.597 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.09 M NPS, 0.1 M imidazole/MES pH 6.5, 50 % MPD_P1K_P3350 |
