9F80
Crystal structure of Rv2242 regulator C-terminal fragment (161-414)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.040, 75.280, 139.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.378 - 2.027 |
| Rwork | 0.196 |
| R-free | 0.25430 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.207 |
| Data reduction software | XDS (20190315) |
| Data scaling software | XDS (20190315) |
| Phasing software | SHELXD (2016/1) |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.378 | 2.150 |
| High resolution limit [Å] | 2.027 | 2.027 |
| Rmeas | 0.073 | 1.353 |
| Number of reflections | 21908 | 3440 |
| <I/σ(I)> | 22.81 | |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 13.4 | 13.5 |
| CC(1/2) | 0.732 | 0.681 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SLOW COOLING | 7.5 | 277 | 20 mM HEPES, pH7.5, 400 mM NaCl, 5% glycerol, 5 mM beta-mercaptoethanol, 400 mM imidazole, temperature 277K, slow cooling |
