9F7K
Glutathione transferase epsilon 1 from Drosophila melanogaster in complex with glutathione
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.14071 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.038, 82.919, 95.543 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.459 - 1.800 |
Rwork | 0.154 |
R-free | 0.17660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.662 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.460 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.054 | 0.812 |
Number of reflections | 60131 | 3542 |
<I/σ(I)> | 17.5 | 2.3 |
Completeness [%] | 99.2 | 99.7 |
Redundancy | 6.6 | |
CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20 % w/v PEG 8000, 100 mM MES pH 6.0 and 200 mM Ca acetate |