9F7J
Rhodococcus diDNase in apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.033200 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.745, 60.353, 134.514 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.280 - 1.720 |
| R-factor | 0.1805 |
| Rwork | 0.178 |
| R-free | 0.20920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.873 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.280 | 1.781 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.175 | 2.034 |
| Rpim | 0.050 | 0.562 |
| Number of reflections | 22757 | 2228 |
| <I/σ(I)> | 11.13 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13.3 | |
| CC(1/2) | 0.997 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 297 | 18% PEG3350, 0.1 M sodium acetate pH 4.5, 0.1 M Bis-Tris pH 5.5 |
