9F7I
Crystal structure of Borrelia burgdorferi B31 CspZ in complex with human FH SCR6-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 44.661, 67.095, 143.602 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.070 - 2.850 |
| R-factor | 0.20402 |
| Rwork | 0.201 |
| R-free | 0.26062 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.647 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.070 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.153 | 0.487 |
| Number of reflections | 11213 | 1580 |
| <I/σ(I)> | 11.1 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.7 | 12.3 |
| CC(1/2) | 0.995 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.25 M ammonium sulfate 0.1 M MES pH 6.5 10% PEG 3000 |
