9F7G

Nocardioides diDNase bound to deoxy-pGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.033220
Spacegroup name	C 2 2 21
Unit cell lengths	35.030, 85.221, 149.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.970 - 1.550
R-factor	0.1456
Rwork	0.143
R-free	0.18000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.746
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.970	1.605
High resolution limit [Å]	1.550	1.550
Rmerge	0.075	0.385
Rpim	0.022	0.182
Number of reflections	32636	2974
<I/σ(I)>	22.03
Completeness [%]	98.9
Redundancy	11.5
CC(1/2)	0.999	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	292	0.2 M lithium sulfate, 0.1 M bis-tris (pH 5.5), 25%(w/v) PEG3350