9F5Q

Crystal structure of selenomethionine-labelled kinetoplastid kinetochore protein KKT23 acetyltransferase domain from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	277
Detector technology	PIXEL
Collection date	2020-12-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9786
Spacegroup name	P 1
Unit cell lengths	42.299, 44.528, 68.969
Unit cell angles	100.54, 103.17, 101.33

Refinement procedure

Resolution	29.530 - 1.950
R-factor	0.19982
Rwork	0.198
R-free	0.22650
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.730
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.530	2.000
High resolution limit [Å]	1.950	1.950
Rmeas	0.110	0.704
Number of reflections	32753	2037
<I/σ(I)>	10.4	2
Completeness [%]	96.5
Redundancy	3.6
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% v/v jeffamine M-2070, 20% v/v DMSO