9F5M
Structure of the C-terminal domain of bacteriophage K gp155, native
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.130, 43.334, 98.982 |
| Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
| Resolution | 62.380 - 1.520 |
| Rwork | 0.188 |
| R-free | 0.21310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.316 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.380 | 1.600 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.063 | 0.647 |
| Rmeas | 0.076 | 0.802 |
| Rpim | 0.042 | 0.466 |
| Number of reflections | 95840 | 13737 |
| <I/σ(I)> | 8.4 | 1.4 |
| Completeness [%] | 95.5 | |
| Redundancy | 2.9 | |
| CC(1/2) | 0.996 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3 | 294 | 15% (w/v) PEG 8000, 83 mM MES-NaOH, 10 mM Tris-HCl |
