9F3Z

Psychrophilic laccase (multicopper oxidase) from Oenococcus oeni 229 with Histag

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	180
Detector technology	PIXEL
Collection date	2021-12-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	I 1 2 1
Unit cell lengths	106.050, 56.690, 182.296
Unit cell angles	90.00, 92.87, 90.00

Refinement procedure

Resolution	93.610 - 3.500
R-factor	0.21008
Rwork	0.208
R-free	0.25727
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.044
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.610	3.830
High resolution limit [Å]	3.500	3.500
Rmerge	0.452	1.230
Rmeas	0.490	1.333
Rpim	0.187	0.510
Total number of observations	93984	22297
Number of reflections	13988	3318
<I/σ(I)>	4.8	2
Completeness [%]	99.9
Redundancy	6.7	6.7
CC(1/2)	0.973	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	50% MPD