Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2021-12-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 88.366, 117.573, 212.531 |
| Unit cell angles | 77.65, 85.34, 70.01 |
Refinement procedure
| Resolution | 44.570 - 2.900 |
| R-factor | 0.2236 |
| Rwork | 0.220 |
| R-free | 0.29470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.255 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.730 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.160 | 0.840 |
| Rmeas | 0.227 | 1.188 |
| Rpim | 0.160 | 0.840 |
| Number of reflections | 167016 | 8505 |
| <I/σ(I)> | 6 | 1.2 |
| Completeness [%] | 95.9 | 97.9 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.985 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 61 mM K/Na tartrate, 18% PEG3350, 12.5 mM EDTA, 100 mM Bis-Tris (pH 7.5) |
