9F3L
Crystal structure of Kluyveromyces lactis glucokinase mutant H304Q in complex with mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 202.412, 88.340, 212.450 |
| Unit cell angles | 90.00, 89.92, 90.00 |
Refinement procedure
| Resolution | 64.280 - 2.480 |
| R-factor | 0.2126 |
| Rwork | 0.211 |
| R-free | 0.23680 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.610 |
| Data reduction software | DIALS (1.dev.877-g8f078f0) |
| Data scaling software | Aimless (0.5.32) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 146.450 | 2.520 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 130184 | 6479 |
| <I/σ(I)> | 7.4 | 0.5 |
| Completeness [%] | 97.9 | 98.8 |
| Redundancy | 4 | 4.2 |
| CC(1/2) | 0.995 | 0.285 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | droplet: 1:1 mixture of protein and reservoir protein supplemented with: 9.8 mM mannose 9.8 mM DTT reservoir solution: 1.6 M sodium malonate pH 6.3 |
