9F3K
Crystal structure of Kluyveromyces lactis glucokinase mutant H304Q in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 4 |
| Unit cell lengths | 205.300, 205.300, 87.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.920 - 2.850 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.23180 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.478 |
| Data reduction software | DIALS (1.dev.877-g8f078f0) |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | REFMAC (5.8.0158) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 145.170 | 2.960 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 42518 | 4410 |
| <I/σ(I)> | 7.5 | 0.6 |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 9 | 9.1 |
| CC(1/2) | 0.993 | 0.282 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | droplet: 1:1 mixture of protein and reservoir protein supplemented with: 9.8 mM glucose 9.8 mM DTT reservoir solution: 1.6 M sodium malonate pH 5.7 |
