9F3J

Crystal structure of Kluyveromyces lactis glucokinase in complex with mannose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91840
Spacegroup name	I 4
Unit cell lengths	187.890, 187.890, 92.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.010 - 2.350
R-factor	0.192
Rwork	0.191
R-free	0.21520
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	0.497
Data reduction software	DIALS (1.dev.877-g8f078f0)
Data scaling software	Aimless (0.5.31)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.970	2.410
High resolution limit [Å]	2.350	2.350
Number of reflections	66871	4469
<I/σ(I)>	7.4	0.9
Completeness [%]	100.0	100
Redundancy	6.6	6.4
CC(1/2)	0.994	0.341

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	droplet: 1:1 mixture of protein and reservoir protein supplemented with: 9.8 mM mannose 9.8 mM DTT reservoir solution: 1.6 M sodium malonate, pH 6.0