9F3I
Crystal structure of Kluyveromyces lactis glucokinase in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91840 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 203.777, 88.108, 212.030 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 64.720 - 2.650 |
| R-factor | 0.2366 |
| Rwork | 0.235 |
| R-free | 0.25760 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.493 |
| Data reduction software | DIALS (1.dev.877-g8f078f0) |
| Data scaling software | Aimless (0.5.31) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 146.800 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 107657 | 5283 |
| <I/σ(I)> | 6.2 | 0.6 |
| Completeness [%] | 98.5 | 98.3 |
| Redundancy | 6.6 | 6.4 |
| CC(1/2) | 0.991 | 0.274 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | droplet: 1:1 mixture of protein and reservoir protein supplemented with: 9.9 mM glucose reservoir solution: 1.5 M sodium malonate, pH 6.3 |
