9F2R

Influenza A/H17N10 polymerase with bound promoter and 3' end of template in active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	91.080, 119.267, 251.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	125.799 - 1.959
Rwork	0.189
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.407
Data reduction software	autoPROC
Data scaling software	autoPROC
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	125.799	2.189
High resolution limit [Å]	1.959	1.965
Rmerge	0.159	1.227
Rmeas	0.166	1.290
Number of reflections	135837	345
<I/σ(I)>	9.7	1.8
Completeness [%]	69.7	68.4
Redundancy	13.3	10.7
CC(1/2)	0.997	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	5 mg/ml polymerase with 5.1 mg/ml (19.5 microM) with 1.14 x molar excess of each RNA (v5' 1-16, v3' 1-18+3 and 15-mer capped primer), mixed in 1:1 ratio of 100 mM amino acids, 100 mM Tris/Bicine pH8.5, 8% ethylene glycol (v/v), 4% PEG 8000 (w/v) by hanging drop at room temperature